In ovo sexing of chicken eggs using Raman imaging and hyperspectral imaging is an innovative method (Link) of determining the sex of chickens before they hatch. This method is more humane and efficient than traditional methods of chicken sexing, which involve physically manipulating the birds or drilling a hole on the surface of eggs via Laser.

Raman imaging is a non-destructive technique that uses a laser to excite the molecules non-invasively in a sample such as the egg shell surface and then measures the scattered light to obtain a chemical fingerprint of the sample. Hyperspectral imaging, on the other hand, captures a wide range of wavelengths of light and can provide detailed information about the chemical composition of egg shell surface and from the inside of the eggs.

Both Raman imaging and hyperspectral imaging can be used to analyze the biochemical composition of chicken eggs to determine the sex of the developing embryos. The imaging data can be analyzed using various big data analytics and data fusion techniques, such as machine learning, to classify the eggs as male or female.

One of the key advantages of this method is that it can be performed in-ovo, meaning that the eggs do not need to be cracked open or otherwise manipulated. This is a significant improvement over traditional methods of chicken sexing, which often involve drilling the egg shell with a 2 to 3 mm hole with a laser and in industrial production physically manipulating the birds, which can cause harm and stress.

The process of in ovo sexing of chicken eggs using Raman imaging and hyperspectral imaging can be broken down into several steps:

  1. Collection of imaging data: The eggs are placed in a chamber or a platform and scanned using Raman imaging and/or hyperspectral imaging to obtain chemical fingerprints of the eggs.
  2. Data preprocessing: The imaging data is preprocessed to remove noise and artifacts, and to prepare it for analysis.
  3. Data analysis: The imaging data is analyzed using big data analytics and data fusion techniques, such as machine learning, to classify the eggs as male or female.
  4. Biomarker identification: The imaging data can be used to identify specific biomarkers that are indicative of the sex of the developing chick.
  5. Recommendation system: A recommendation system can be developed via Artificial Intelligence and then used to provide an overall sex prediction for each egg, taking into account the results of the data analysis and biomarker identification.

In Ovo sexing of chicken eggs using Raman imaging and hyperspectral imaging is a promising method of determining the sex of chickens before they hatch. It is more humane and efficient than traditional methods of chicken sexing and can be performed non-invasively, without cracking open the eggs. With the use of big data analytics, data fusion and recommendation system, this method can be even more accurate and efficient.